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Anti-tubercular and antioxidant screening of Annona Reticulata Linn. and Borassus Flabellifer Linn.

Volume No : (2020) Volume: 08 Issue : 39 Year : 2020 Page No: 89-96

Authors : Ganesh S. Tolsarwad,Prasad G. Jamkhande, Mahesh M. Biradar, Shrikrushna A. Shinde

Abstract :

Background: The Borassus flabellifer Linn. and Annona reticulate Linn. plant parts such as leaves, fruit, bark, and seed have been reported for possessing anti-inflammatory, antiarthritic activity, and antibacterial activity. This work was, therefore, designed to examine an antioxidant and antimicrobial activities of methanol extract and chloroform, acetone, and n-butanol fractions.
Materials and Methods: Antioxidant activity was determined by the 1, 1-diphenyl-2-picrylhydrazyl (DPPH), H2O2, radical scavenging assay, total antioxidant activity, and Fe3+ reducing power assay. The anti-tubercular activity was investigated by both the agar well and disk diffusion method by three strains of mycobacteria, MIC.
Results: Antioxidant activity of methanol extract, chloroform fractions, n-butanol fraction, acetone fraction of Borassus flabellifer Linn., and methanolic extract of
Annona reticulate Linn. were found to inhibit DPPH and hydrogen peroxide free radicals as 40–59%, 45–60%, 47–68%, and 39–65%, for methanolic extract of Annona reticulate Linn. and 48–68%, 28–61%, 43–75%, 31–46%, and 37–80% for methanolic extract of Annona reticulate Linn. 12–75%. While, total antioxidant result was found between 1% and 33%, 1% and 40%, 2% and 42%, and 16% and 47% for methanolic extract of Annona reticulate Linn. 9%–17%. Potential of methanol extract and fractions for reducing of Fe3+ was found fairly moderate when compared with ascorbic acid as standard.
Conclusion: Results clearly reveal that the extract and fractions is found to possess considerable significant activity against mycobacterial infections and found to have deteriorating potential in oxidative damage induced by free radicals to the cell and cell components.

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