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Development and validation of a novel isocratic RP-HPLC method   for simultaneous determination of 6-gingerol and  thymol in herbal formulation NBIOTIC Premix

Volume No : (2020) Volume: 08 Issue : 38 Year : 2020 Page No: 39-44

Authors : Kotagiri Ravikanth, Anirudh Sharma, Deepak Thakur, Pushap Lata

Abstract :

A high-performance liquid chromatography method employing diode array detection was developed to determine levels of the 6-gingerol and thymol simultaneously, which are present in a natural complex matrices found in ayurvedic commercial product NBIOTIC Premix. Effective chromatographic separation of 6-gingerol and thymol was achieved using a Phenomenex Luna5µ (RP C18, 25 cm × 4.6 mm × 100Å) column with isocratic elution of the mobile phase composed of acetonitrile and water in 50:50 ratio. The proposed HPLC method was statistically validated with respect to linearity, range, precision, accuracy, selectivity, and robustness. Calibration curves were linear in the ranges of 6.0–48.0 µg/ml for 6-gingerol and 15.0–75.0 µg/ml thymol, respectively, with correlation coefficients >0.996. The HPLC method was applied to herbal formulation NBIOTIC Premix, in which the phytoconstituents were successfully quantified with good recovery values with no interfering peaks from the excipients. 

Keywords: 6-Gingerol, HPLC, NBIOTIC Premix, phytoconstituents, polyherbal, simultaneous, thymol  

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