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In vitro protein denaturation inhibition assay of Eucalyptus globulus and Glycine max for potential anti-inflammatory activity

Volume No : (2020) Volume: 08 Issue : 38 Year : 2020 Page No: 28-31

Authors : Nayana Bhikaji Mirke, Pallavi Sitaram Shelke, Poonam Ramachandra Malavdkar, Pradnya Nilesh Jagtap

Abstract :

Background: Eucalyptus globulus and Glycine max (G-max) are well known and widely used herbs, which contain several interesting bioactive constituents and possess health-promoting properties. To reduce the use of animals in conducting anti-inflammatory activity, in vitro studies as animal ethics is important as human welfare.

Aim:The present work was carried out to evaluate the potential anti-inflammatory property of the combination effect of E. globulus + G-max extracts against the denaturation of protein in vitro using egg and bovine serum albumin (BSA) method. Materials and Methods: The test extract, at different concentrations, was incubated with egg and BSA under controlled experimental conditions and subjected to determination of absorbance at 660 nm to evaluate the anti-inflammatory property. Methotrexate was used as the reference standard drug.

Results:The present results exhibited a concentration-dependent inhibition of protein (albumin) denaturation by the test extract. The outcome of methotrexate was found to be less effective when compared with the test extract.

Conclusion: From the present findings, it can be concluded that the E. globulus + G-max possessed a potent anti-inflammatory effect against the denaturation of protein in vitro. This effect could be either due to the synergistic activity of these two herbal drug combinations.

Keywords: Anti-inflammatory potential, eucalyptus oil, glycine max, protein denaturation

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