Volume No : (2015) Volume: 03 Issue : 15 Year : 2015 Page No: 536-543
Authors : Nitin B. Ghiware, Mahavir H. Ghante and Rajendra M. Kawade
Abstract :
Woodfordia fruticosa (WF) flower extracts (Petroleum ether- PEWF; Chloroform - CHWF; Ethyl acetate- EAWF; Methanol- MEWF; Hydroalcohol - HAWF) was evaluated for the phytochemical contents using phytochemical screening and TLC fingerprinting that was standardized for phytoconstituents by spectroscopic methods. Phytoconstituents of the extracts were validated using HPLC for specificity by comparing with standard retention time, following which was further screened for in vitro DPPH scavenging activity and concurrently for in vivo cardioprotective activity against doxorubicin induced cardiotoxicity in rats. WF flower extracts showed presence of polyphenols, triterpenoids, sterols and cardiac glycosides which was affirmed by TLC. Extracts were spectroscopically standardized for the content of total polyphenols (MEWF> EAWF> HAWF) and flavonoid contents (MEWF> EAWF> HAWF). Standardization performed by comparative retention time with standard phytoconstituents using HPLC revealed the presence of gallic, catechin, ellagic, rutin along with chlorogenic acid and caffeic acid. Extracts that had significant content of polyphenols and flavonoids reflected comparatively good % DPPH scavenging activity (MEWF - 96.7; EAWF - 95.6; HAWF - 75.5 at 50μg / ml). All the extracts also revealed safe dose margin when subjected to acute oral toxicity. Further, in vivo cardioprotective evaluation of MEWF and EAWF extracts in doxorubicin induced cardiotoxicity in rats revealed significant preventive activity (P<0.05) against elevation in serum markers as compared to that in doxorubicin treated animals. MEWF extract shown combined potential of inhibition of doxorubicin induced elevation in serum level marker and better % DPPH scavenging activity as compared to EAWF extract.
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