Aim: Isolation of bergenin from Bergenia ciliata, evaluation for antioxidant activity, and docking studies.
Materials and Methods: Column chromatography was performed for the isolation of bergenin from B. ciliata aqueous extract and characterized by nuclear magnetic resonance. Antioxidant activity of bergenin was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and docking studies of bergenin were performed against molecular targets relevant to urinary ailments.
Results: The phenolic nature of bergenin improves solubility in aqueous solution; isolation of bergenin from aqueous extract was performed by column chromatography and purified bergenin was evaluated for antioxidant activity using DPPH assay. It was showing mild-to-moderate antioxidant action, whereas docking studies of bergenin against pathogens target showing good ligand-binding interaction.
Conclusion: Isolation of bergenin from aqueous extract was reported 1st time and showing mild-to-moderate antioxidant action. Moreover, docking studies of bergenin against different pathogens show promising ligandbinding interaction.
Keywords: Antioxidant, Bergenia ciliata, bergenin, characterization, docking studies, isolation