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To determine antifungal susceptibility of dermatophyte isolates in a tertiary care hospital using microdilution method: A prospective cohort study

Volume No : (2018) Volume: 06 Issue : 30 Year : 2018 Page No: 21-26

Authors : M. Suganthi, V. Dillirani, P. R. Thenmozhivalli, R. Selvi, B. J. Anand

Abstract :

Aim: The aim of the study was to standardize in vitro antifungal susceptibility testing by microbroth dilution method to find out the minimum inhibitory concentration (MIC) of fluconazole, ketoconazole, itraconazole, terbinafine, and griseofulvin fungal isolates of skin, hair, and nail. Materials and Mehods: Various samples were collected from patients with clinically diagnosed dermatophytosis. Skin scrapings, hair, and nail were collected from 170 patients. Results: The antifungal drugs such as fluconazole (0.5–0.16 μg/ml), ketoconazole (0.03–0.5 μg/ml), itraconazole (0.007–0.25 μg/ml), terbinafine (32–0.0313 μl/ml), and griseofulvin (0.03–0.25 μg/ml) these water-insoluble drugs were incorporated in dissolved in dimethyl sulfoxide. The MIC range, MIC 50, and MIC 90 for the drug griseofulvin were found to be 0.03–0.25, 0.06, and 0.12, respectively, for ketoconazole were found to be 0.03–0.5, 0.12, and 0.5, respectively, for the drug fluconazole were found to be 1–32, and 1, respectively, itraconazole were found to be 0.007–0.06, 0.03, and 0.06, respectively, and for terbinafine were found to be 0.007–0.06, 0.015, and 0, respectively. This technique was found to be reliable, cost-effective, and easy to perform with consistent results. Conclusion: Further, results concluded that the itraconazole showed a higher MIC value when compared to other antifungal drugs.

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