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Research Article
Year : 2014   |  Volume : 2   |  Issue : 4   |  Page : 425-436  

Combined effect of baclofen and acamprosate in experimental models of peripheral neuropathic pain in Wistar rats

Varun Vikas Vija, Gurmanpreet Kaura, Onkar Bedia, Vijay Kumara, Sumit Jamwalb, Puneet Kumara, Rahul Deshmukha

Correspondence Address:Department of Pharmacology, I.S.F College of Pharmacy, Moga-142001, Punjab, India. Research Scholar, Punjab Technical University, Jalandhar, Punjab

Source of Support: nil, Conflict of Interest: not declared


DOI: 10.4103/2231-4040.197331

Abstract  

Objective: Neuropathic pain (NP) is defined as pain associated with damage or permanent alteration of the peripheral or central nervous system. Current drug treatment for the management of neuropathic pain associated with various adverse effects. The present study was designed to investigate the combined effect of acamprosate and baclofen in experimental model of peripheral neuropathic pain in wistar rats. Material and methods: Neuropathic pain was induced by Chronic constriction injured (CCI) of sciatic nerve in rats. Acamprosate (100 and 200 mg/kg p.o) and baclofen (10 and 20 mg/kg p.o) was given in different groups for 14 days starting on 7th day post sciatic nerve ligation. Further combination of acamprosate(100 mg/kg p.o) and baclofen (10 mg/kg p.o) was also given to one group. On 1th, 3rd, 7th, 14thand 21stday behavioral parameters like mechanical allodynia and thermal hyperalgesia were assessed. Then animals were sacrificed on 22nd day and biochemical parameters (GSH, LPO, catalase, nitrite, SOD) were assessed. Results: Ligation of sciatic nerve significantly induced mechanical allodynia and thermal hyperalgesia with increase in oxidative stress (increase in LPO and nitrite) and decline of anti-oxidant enzyme levels (catalase, SOD, GSH) in sciatic nerve homogenate. Acamprosate (100 and 200 mg/kg p.o) and baclofen (10 and 20 mg/kg p.o) attenuated all the behavioural and biochemical parameters alone and/or combination. Conclusion: On the basis of obtained data, it can be concluded that acamprosate and baclofen alone and combination significantly prevented the development of NP in rats by exhibiting anti-hyperalgesic and anti-nociceptive effects, decreasing oxidative stress and increasing the anti-oxidant capacity.

Keywords: Baclofen, acamprosate, neuropathic pain, allodynia, hyperalgesia, oxidative stress

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