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Research Article
Year : 2017   |  Volume : 5   |  Issue : 4   |  Page : 190-194  

To determine antifungal susceptibility for the isolates using agar dilution method: A prospective cohort study

M. Suganthi, V. Dillirani, P. R. Thenmozhivalli, R. Selvi, B. J. Anand

Correspondence Address:Department of Microbiology, Government Kilpauk Medical College, Chennai, Tamil Nadu, India

Source of Support: nil, Conflict of Interest: not declared


DOI: 10.4103/2231-4040.197331

Abstract  

The aim of the study was to standardize in vitro antifungal susceptibility testing by agar dilution method to find out the minimum inhibitory concentration (MIC) of fluconazole, ketoconazole, itraconazole, terbinafine, and griseofulvin fungal isolates of skin, hair, and nail. Various samples were collected from patients with clinically diagnosed dermatophytosis. Skin scrapings, hair, and nail were collected from one hundred and seventy patients. The antifungal drugs such as fluconazole (64–0.0625 μl/ml), ketoconazole (32–0.0313 μl/ml), itraconazole (32–0.0313 μl/ml), terbinafine (32–0.0313 μl/ml), and griseofulvin (32–0.0313 μl/ml) these water-insoluble drugs were incorporated in dissolved in dimethyl sulfoxide. The MIC was determined as the lowest concentration of the antifungal drug preventing the growth of macroscopically visible colonies on drug-containing plates when there was visible growth on the drug-free control plates. The MIC range, MIC 50, and MIC 90 for the drug griseofulvin were found to be 0.06–0.1, 0.25, and 1, respectively, for ketoconazole were found to be 0.06–0.1, 0.5, and 1, respectively, for the drug fluconazole were found to be 1–32, 8, and 16, respectively, itraconazole were found to be 0.06–4, 0.5, and 1, respectively, and for terbinafine were found to be 0.03–0.12, 0.06, and 0.12, respectively. This technique was found to be reliable, cost-effective and easy to perform with consistent results. Further results concluded that the fluconazole showed a higher MIC value when compared to other antifungal drugs.

Keywords: Agar dilution method, antifungal susceptibility testing, fluconazole, itraconazole, ketoconazole, minimum inhibitory concentration, terbinafine and griseofulvin

How to cite this article:
Suganthi M, Dillirani, Thenmozhivalli, Selvi, Anand BJ. To determine antifungal susceptibility for the isolates using agar dilution method: A prospective cohort study. Innov Pharm Pharmacother 2017;5(4):190-194.

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